Similiar to Doofenshmirtz, Charlene has the most personality differentiation from her original counterpart. As opposed to being good-natured and sarcastic, Charlene is very evil and intelligent, sharing much of her husband's evilness. At one time, she was able to formulate an attack at Danville Park as a successful diversion in order for Candace to bring Heinz to her penthouse in order to free him. She also proved to be a highly skilled martial art fighter, as she was able to disarm Candace and hold against her with a Bo staff.
Just like her husband, Charlene is actually also willing to eliminate those that pose as some significant obstacle, such as blasting a grenade at the Firestorm Girls when they arrived at her lair.
Charlene seems to care for her looks, evident when was battling Candace, she notices one of the freed agents cutting off her manicure, something which distracted Charlene long enough for Candace to defeat her in combat.
Despite her being a darker and more sinister version of the 1st Dimension Charlene, she does tends to have a lighter side. As such, she still loves Heinz, since they had pretended to be divorced for several advantages during Heinz's reign. Also just like her original counterpart, she cares for their daughter Vanessa, who was willing enough to help both her and Heinz escape from Resistance custody.